|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||0.03-0.1 ug/ml|
|Immunocytochemistry (ICC)||See 1 publications below|
|Tested Species reactivity||Human, Pig|
|Published species reactivity||Pig|
|Host / Isotype||Goat / IgG|
|Immunogen||Synthetic peptide sequence (QNQRMKSKRWQKNN) corresponding to the internal amino acids of NANOG|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
This antibody is predicted to react with canine based on sequence homology.
This antibody has been successfully tested in a Sandwhich ELISA as both a capture and a detection antibody in combination with PA5-18678.
Expression of NANOG is required for the maintenance of pluripotency in epiblast and embryonic stem (ES) cells as well as for the ability to maintain ES self-renewal independently of LIF/Stat3. The role of NANOG in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (also known as Oct-4), another germline-specific transcription factor, and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: HGNC:20857; hNanog; Homeobox protein NANOG; Homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; homeodomain transcription factor Nanog; NANOG; Nanog homeobox
Gene Aliases: NANOG
UniProt ID: (Human) Q9H9S0
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