|ChIP assay (ChIP)||Assay-Dependent|
|Flow Cytometry (Flow)||Assay-Dependent|
|Immunoprecipitation (IP)||3 ul|
|Tested Species reactivity||Hamster, Human, Mouse, Non-human primate|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Partial length human NBS1 protein [Swiss-Prot #O60934]|
|Storage buffer||whole serum|
|Contains||0.02% sodium azide|
|Storage conditions||4° C, do not freeze|
ICC/IF has been performed using methanol-fixed IMR90 primary human fibroblasts.
Suggested positive control: antigen standard for NBN (transient overexpression lysate), TK6 and HeLa whole cell extract.
The p95 gene (identical to NBS1 and nibrin) is a member of the hMre11/hRad50 double-strand break complex. This protein complex has been implicated in Nijmegen breakage syndrome, an autosomal recessive disorder marked by increased cancer incidence, cell cycle checkpoint deficits, and ionizing radiation sensitivity, thus revealing a direct molecular link between double-strand break repair and cell cycle checkpoint functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: AT V1; AT V2; ATV; Cell cycle regulatory protein p95; NBS; Nibrin; Nijmegen breakage syndrome; Nijmegen breakage syndrome 1 (nibrin); Nijmegen breakage syndrome protein 1; Nijmegen breakage syndrome protein 1 homolog; p95 protein of the MRE11/RAD50 complex
Gene Aliases: AT-V1; AT-V2; ATV; I79_012607; NBN; NBS; NBS1; P95
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.