|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein encoding aa119-276 of human NCK2|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is tonsil or breast carcinoma.
The NCK family of SH2/SH3 adaptor proteins consists of two members, NCK1 and NCK2, which couple tyrosine kinase signaling, including the EGF and PDGF receptor-pathways, to downstream signaling proteins. The NCK2 protein binds human PDGFR-b (Tyr-1009); overexpression of NCK2 inhibits PDGF-induced membrane ruffling and lamellipod formation.
Protein Aliases: beta; cytoplasmic protein NCK2; GRB4; growth factor receptor-bound protein 4; NCKbeta; noncatalytic region of tyrosine kinase; noncatalytic region of tyrosine kinase, beta; SH2/SH3 adaptor protein NCK-beta
Gene Aliases: GRB4; NCKbeta
UniProt ID: (Human) O43639
Entrez Gene ID: (Human) 8440