|ChIP assay (ChIP)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||4 µg/ml|
|Immunocytochemistry (ICC)||See 1 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide corresponding to residues C G G A(2016) S A S D P H R E K T Q(2027) of human SMRT.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA1-843 detects silencing mediator of retinoic acid and thyroid hormone receptor (SMRT) from human cells and tissues.
MA1-843 has been successfully used in Western blotting and immunohistochemical procedures. By Western blot, this antibody detects ~250 kDa and ~220 kDa proteins representing endogenous levels of both known isoforms of SMRT in HeLa cell extracts. This antibody does not react with mouse tissue in Western blot applications.
The MA1-843 immunogen is a synthetic peptide corresponding to residues C G G A(2016) S A S D P H R E K T Q(2027) of human SMRT. MA1-843 immunizing peptide (Cat. # PEP-043) is available for use in neutralization and control experiments.
Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand-dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate. Silencing mediator of retinoic acid and thyroid hormone receptor (SMRT) and nuclear receptor corepressor (N-CoR) are related transcriptional corepressors which contain two distinct domains capable of interacting with unliganded nuclear receptors to repress their basal transcriptional activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CTG repeat protein 26; N-CoR2; Nuclear receptor corepressor 2; silencing mediator for retinoid and thyroid hormone receptors; Silencing Mediator of Retinoic Acid and Thyroid Hormone Receptor; Silencing mediator of retinoic acid and thyroid hormone receptor; SMAP270; SMRT; T3 receptor-associating factor; Thyroid-, retinoic-acid-receptor-associated corepressor; TRAC
Gene Aliases: CTG26; N-CoR2; NCOR2; SMAP270; SMRT; SMRTE; SMRTE-tau; TNRC14; TRAC; TRAC-1; TRAC1
UniProt ID: (Human) Q9Y618
Entrez Gene ID: (Human) 9612
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