|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:10,000|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 312 and 642 of Human NDUFS1|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA5-28220 targets NDUFS1 in Western blot and immunohistochemistry (Paraffin) applications and shows reactivity with Human, Rat and mouse samples.
The PA5-28220 immunogen is recombinant fragment corresponding to a region within amino acids 312 and 642 of Human NDUFS1.
The protein encoded by this gene belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Mutations in this gene are associated with complex I deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 75-kD subunit; CI-75k; CI-75Kd; complex I; complex I 75kDa subunit; complex I, mitochondrial respiratory chain, 75-kD subunit; Complex I-75kD; mitochondrial; mitochondrial NADH-ubiquinone oxidoreductase 75 kDa subunit; mitochondrial respiratory chain; NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa; NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase); NADH-ubiquinone oxidoreductase 75 kDa subunit; NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial; PRO1304
Gene Aliases: 5830412M15Rik; 9930026A05Rik; CI-75k; CI-75Kd; NDUFS1; PRO1304
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