|Immunohistochemistry (Paraffin) (IHC (P))||Assay-Dependent|
|Western Blot (WB)||1:500-1:3000|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment corresponding to a region within amino acids 185 and 349 of Human NEK2|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA5-31259 targets NEK2 in IF and WB applications and shows reactivity with Human samples.
The PA5-31259 immunogen is recombinant protein fragment corresponding to a region within amino acids 185 and 349 of Human NEK2.
Protein kinase that is involved in mitotic regulation. Integral component of the mitotic spindle-assembly checkpoint which is necessary for proper chromosome segregation during metaphase-anaphase transition. Required for association of MAD2L1 to kinetochore. Phosphorylates SGOL1. May have a role at the G2-M transition. May also play a role in meiosis. Isoform 1 but not isoform 2 appears to play a role in centrosome splitting. Isoform 1 phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2, which is not present in the nucleolus, does not.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: HSPK 21; HsPK21; NEK2A; Never in mitosis A-related kinase 2; NIMA (never in mitosis gene a)-related kinase 2; NimA-like protein kinase 1; NIMA-related kinase 2; NimA-related protein kinase 2; NLK1; protein phosphatase 1, regulatory subunit 111; Serine/threonine-protein kinase Nek2
Gene Aliases: HsPK21; NEK2; NEK2A; NLK1; PPP1R111; RP67
UniProt ID: (Human) P51955
Entrez Gene ID: (Human) 4751
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