|Gel Shift (GS)||Assay dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Immunoprecipitation (IP)||3 µg|
|Western Blot (WB)||1:1,000|
|Western Blot (WB)||See 13 publications below|
|ChIP assay (ChIP)||See 1 publications below|
|Immunohistochemistry (IHC)||See 2 publications below|
|Tested Species reactivity||Human, Mouse|
|Published species reactivity||Rabbit , Rat , Human , Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues C D(1439) L L V S L Q N Q G N N L T G S F(1455) of human NFAT5.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-023 detects nuclear factor of activated T-cells 5 (NFAT5) from human and mouse cells as well as recombinant human protein.
PA1-023 has been successfully used in Western blot, immunofluorescence, immunoprecipitation, gel shift and immunocytochemistry procedures. By Western blot, this antibody detects an ~170 kDa protein representing NFAT5 in HEK293 cells transfected with the human NFAT5 gene. Immunocytochemical staining of NFAT5 in HEK293 cells transfected with the human NFAT5 gene with PA1-023 results in primarily cytoplasmic staining.
The PA1-023 immunogen is a synthetic peptide corresponding to residues C D(1439) L L V S L Q N Q G N N L T G S F(1455) of human NFAT5. This sequence is 94% conserved in the mouse protein. PA1-023 immunizing peptide (Cat. # PEP-123) is available for use in neutralization and control experiments.
The nuclear factor of activated T-cells (NFAT) transcription complex is required for the expression of a group of proteins that collectively regulate the immune response. Four NFAT proteins, encoded on separate genes and expressed as several splice variants, have been described: NFAT1 (also known as NFATp or NFATc2), NFAT2 (NFATc or NFATc1), NFAT3, and NFAT4 (NFATx or NFATc3). These proteins show a low level of sequence similarity with the Dorsal/Rel/NFkB family of transcription factors. Another NFAT-related protein termed NFAT5 differs from isoforms 1-4 in that it lacks many of the Fos/Jun contact sites observed in its predecessors and its subcellular localization is not calcineurin-dependent.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: glutamine rich protein H65; NF-AT5; NFAT-like protein 1; NFATL1; Nuclear Factor Activated T-Cells; Nuclear factor of activated T cells 5; Nuclear factor of activated T-cells 5; osmotic response element-binding protein; Rel domain-containing transcription factor NFAT5; T cell transcription factor NFAT5; T-cell transcription factor NFAT5; TonE-binding protein; Tonicity-responsive enhanc; tonicity-responsive enhancer binding protein; Tonicity-responsive enhancer-binding protein
Gene Aliases: AI225870; B130038B15Rik; CAG-8; CAG80; KIAA0827; mKIAA0827; NF-AT5; NFAT5; NFATL1; NFATZ; OREBP; TONEBP
Molecular Function: transcription factor
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