|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment corresponding to a region within amino acids 1 and 347 of Human NFYA|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA5-28990 targets NFYA in IF, IHC (P), and WB applications and shows reactivity with Human and mouse samples.
The PA5-28990 immunogen is recombinant protein fragment corresponding to a region within amino acids 1 and 347 of Human NFYA.
The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: A subunit; CAAT box DNA-binding protein subunit A; CAAT-box DNA binding protein subunit A; CAAT-box DNA-binding protein subunit A; CCAAT-binding transcription factor subunit B; HAP2; HAP2 CCAAT-binding protein; NF-YA; nuclear transcription facto; Nuclear transcription factor Y subunit A; Nuclear transcription factor Y subunit alpha; nuclear transcription factor Y, alpha; Transcription factor NF-Y; Transcription factor NF-Y, A subunit
Gene Aliases: AA407810; CBF-A; CBF-B; HAP2; NF-YA; NFYA; SEZ-10; SEZ10
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