|ELISA (ELISA)||0.1-1.0 ug/ml|
|Flow Cytometry (Flow)||3-5 µg/million cells|
|Gel Shift (GS)||5-10 ug/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20-1:200|
|Western Blot (WB)||1.0 ug/ml|
|Western Blot (WB)||See 4 publications below|
|Tested Species reactivity||Human, Mouse|
|Published species reactivity||Rat , Virus Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein derived from an internal region of the human NF-kappa-B (p50) (nuclear factor of kappa-B).|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
NF-kB is a nuclear transcription factor activated by various extra and intracellular stimuli such as cytokines, UV radiation, stress, in injury, and by bacterial and viral products. It is involved in regulation of various cellular events including cell growth, differentiation, proliferation, apoptosis and inflammation. NFKB1 (p50), a 50KDa functional sub-unit of NF-kB, is a member of Rel protein family. It is synthesized as a p105 precursor protein and consists of an N-terminal conserved RHD-region containing nuclear localization signal, DNA-binding and dimerization domains. NFKB1 (p50) forms homodimers or heterodimerizes with p65, forming the functional NF-kB factor. NFKB1 (p50) directs the nuclear translocation of NF-kB and is instrumental in its DNA-bidning. Pathological role of NF-kB has been suggested in AIDS, hematogenic cancer cell metastasis and rheumatoid arthritis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DNA-binding factor KBF1; EBP-1; NF kappaB1; NF-kappa-B1 p84/NF-kappa-B1 p98; NF-kappaB p50; NF-kappabeta; NFkB-p50; NFKB1; nuclear factor kappa-B DNA binding subunit; nuclear factor kappaB p50; Nuclear factor NF-kappa-B p105 subunit; nuclear factor NF-kappa-B p50 subunit; nuclear factor of kappa light chain gene enhancer in B-cells 1, p105; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1; nuclear factor of kappa light polypeptide gene enhancer in B-cells 1, p105; nuclear factor-kappaB p50; p50 subunit of NF kappaB; p50 subunit of NF-kappaB
Gene Aliases: CVID12; EBP-1; KBF1; NF-kappa-B; NF-kappaB; NF-kappaB1; NF-KB1; NFkappaB; NFKB-p105; NFKB-p50; NFKB1; p105; p50; p50/p105
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