|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from N-terminus of human NFkB p50|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is tonsil, testis or prostate tissue.
NF kappa B is a heterodimer consisting of a 50kDa DNA binding subunit and a 65kDa transactivating subunit (RelA). P50 subunit of NFkB has an isoform and both the proteins of 105 and 100kDa respectively. The p50/p65 heterodimer is present in the cytoplasm in active form bound to its inhibitor, IkB. Upon stimulation of cells with stimuli like LPS, Interlekin-1, IkB gets phosphorylated and degraded by proteosomes releasing the activated NFkB which goes to the nucleus inducing gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DNA binding factor KBF1; DNA-binding factor KBF1; EBP-1; KBF1; NF-kappa-B; NF-kappaB; NF-kappabeta; NF-kB1; NFkappaB; NFKB-p105; NFKB-p50; nuclear factor kappa-B DNA binding subunit; Nuclear factor NF-kappa-B p105 subunit; nuclear factor NF-kappa-B p50 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
Gene Aliases: CVID12; EBP-1; KBF1; NF-kappa-B; NF-kappaB; NF-kB1; NFkappaB; NFKB-p105; NFKB-p50; NFKB1; p105; p50
UniProt ID: (Human) P19838
Entrez Gene ID: (Human) 4790
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