|Immunocytochemistry (ICC)||4-6 ug/ml|
|Immunofluorescence (IF)||4-6 ug/ml|
|Western Blot (WB)||1:100-1:1000|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A recombinant protein corresponding to amino acids 706-860 of Q99558.|
|Contains||0.09% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
This antibody is predicted to react with Rhesus monkey, mouse, rat, bovine, equine and chicken based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
NIK, also known as mitogen-activated protein kinase kinase kinase (MAP3K) 14 is an NF-kappaB inducing protein kinase of the serine/threonine kinase family. NIK binds to Traf2 to activate NF-kappaB as part of a signaling cascade involving receptors of the TNF/NGF family and the interleukin-1 type-I receptor. NIK protein is shuttled between cytosolic, nuclear and nucleolar compartments. When shuttled to the nucleoli, NIK-dependant NF-kappaB activation is impaired relative to its nuclear localization revealing a possible means of regulation. Reactive oxygen species can interact directly with NIK to alter NF-kappaB activation. Additionally, NIK specifically interacts with Down syndrome candidate region 1 (DSCR1) blocking its proteosomal degradation leading to DSCR1 accumulation and cytotoxicity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: FTDCR1B; HS; HSNIK; Mitogen-activated protein kinase kinase kinase 14; NF-kappa-beta-inducing kinase; NIK; serine/threonine protein-kinase; Serine/threonine-protein kinase NIK
Gene Aliases: FTDCR1B; HS; HSNIK; MAP3K14; NIK
UniProt ID: (Human) Q99558
Entrez Gene ID: (Human) 9020
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