|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1µg/ml|
|Tested Species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Baculovirus-expressed recombinant human NGFI-Ba (aa 2-249)|
|Purification||Ammonium sulfate precipitation|
|Storage buffer||physiological saline|
|Contains||0.1% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
This antibody specifically recognizes human NGFI-B-alpha but does not recognize human NGFI-B-beta or gamma.
NR4A1 is a member of the steroid-thyroid hormone-retinoid receptor superfamily. NGFI-B-alpha is expressed in brain, adrenal, thyroid, liver, testis, ovary, thymus, muscle, lung and prostate. Its expression is induced in response to various stress stimuli and growth factors. The encoded protein acts as a nuclear transcription factor involved in mediating apoptotic signaling in thymocytes and tumor cells, as well as having a role in signaling in the hypothalamic-pituitary axis. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Three transcript variants encoding two distinct isoforms have been identified for this NR4A1. Variant (3), also known as TRC beta, contains multiple differences compared to variant 1. The resulting isoform b contains a shorter and distinct C-terminus compared to isoform a. RXR has been shown to be a partner for NR4A1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Early response protein NAK1; GFRP1; growth factor-inducible nuclear protein N10; HMR; hormone receptor; N10; NAK-1; NAK1; Nerve Growth Factor I-B-alpha; Nerve Growth Factor I-Ba; nerve growth factor IB nuclear receptor variant 1; Nerve Growth Factor Induced Clone B Alpha; NGFIB; NP10; NR4A1; Nuclear hormone receptor NUR/77; Nuclear receptor subfamily 4 group A member 1; nuclear receptor subfamily 4, group A, member 1; Nur77; Orphan nuclear receptor HMR; Orphan nuclear receptor TR3; Orphan receptor tr3; Receptor NGFIB; ST-59; Steroid receptor TR3; Testicular receptor 3; TR3; TR3 orphan receptor
Gene Aliases: GFRP1; HMR; N10; NAK-1; NAK1; NGFIB; NP10; NR4A1; NUR77; TR3
UniProt ID: (Human) P22736
Entrez Gene ID: (Human) 3164
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