|Immunohistochemistry (Paraffin) (IHC (P))||Assay-Dependent|
|Western Blot (WB)||1:500|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A peptide derived from the human NTH1 conjugated to KLH.|
|Purification||Antigen affinity chromatography|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
The target sequence has 93% sequence homology with bovine proteins.
Suggested positive control: Hela whole cell lysate, K562 cell extracts, Hela cell extracts, K562 whole cell lysate.
Oxygen radicals damage chromosonal DNA causing cell death and inducing mutations. Although a major focus of oxidatively damaged DNA has centered on the repair of 8-oxo-G, a number of other damaged DNA sites are created by free-radical attack on DNA. The human NTH1 repair protein is one enzyme that has been shown to act on a large number of these other oxidatively damaged DNA sites.A homologue of E. coli Endonuclease III, NTH is a DNA glycosylase with apurinic/apyrimidinic lyase activity. The NTH protein has broad substrate specificity, including numerous ring saturation and fragmentation products of pyrimidines. Research indicates NTH plays a crucial role in removal of oxidative base lesions in mitochondrial DNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: bifunctional DNA N-glycoslyase/DNA-(apurinic or apyrimidinic site) lyase; Bifunctional DNA N-glycosylase/DNA-(apurinic or apyrimidinic site) lyase; DNA glycoslyase/AP lyase; DNA glycosylase/AP lyase; Endonuclease III like 1; Endonuclease III-like protein 1; hNTH1; nth endonuclease III-like 1; NTHL 1; OCTS 3; thymine glycol DNA glycosylase/AP lyase
Gene Aliases: FAP3; hNTH1; NTH1; NTHL1; OCTS3
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