|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:200|
|Western Blot (WB)||1:500-1:1000|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to amino acids 432-486 of human Nephrocystin-5|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2|
|Contains||0.05% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
This antibody detects endogenous protein at a molecular weight of 69 kDa.
Purity is >95% by SDS-PAGE.
The nephrocystin proteins comprise a family of five enzymes that commonly interact with p130Cas, proline-rich tyrosine kinases, calmodulin and tensin, indicating that these proteins may participate in a common signaling pathway. Localized to the outer segments and primary cilia of photoreceptor cells, nephrocystin-5 is complexed with RPGR (retinitis pigmentosa GTPase regulator) and interacts directly with calmodulin. Nephrocystin-5 is thought to participate with RPGR in a pathway of ciliary function in the kidey and retina. Mutations in the gene encoding nephrocystin-5 are the primary cause of Senior-Loken syndrome 5, a juvenile disorder characterized by defects in the waste filtering system of the kidney, as well as retinal degradation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: IQ calmodulin-binding motif-containing protein 1; IQCB1; KIAA0036; nephrocystin 5; Nephrocystin-5; NPHP5; OK/SW-cl.85; p53 and DNA damage-regulated IQ motif protein; PIQ
Gene Aliases: 6820449I09Rik; AV128382; IQCB1; KIAA0036; NPHP5; OK/SW-cl.85; PIQ; SLSN5
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