|Flow Cytometry (Flow)||1 ug/test|
|Immunocytochemistry (ICC)||1:100 - 1:250|
|Immunofluorescence (IF)||1:20 - 1:250|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20-1:100|
|Western Blot (WB)||1:500 - 1:1500|
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Tested Species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Human nestin protein corresponding to residues 1464-1614|
|Storage buffer||PBS with 1mg/ml BSA, 30% glycerol|
|Contains||0.05% sodium azide|
MA1-110 has been successfully utilized in Western Blot, immunocytochemistry, flow cytometry, and immunofluorescence applications in human samples. MA1-110 also acts a neuronal stem cell marker and detects a band in Western blot at ~250 kDa.
Nestin is expressed by many types of cells during development, although its expression is usually transient and does not persist into adulthood. One instance of nestin expression in adult organisms, and perhaps that for which nestin is best known, are the neuronal precursor cells of the subgranular zone. Nestin is an intermediate filament protein expressed in dividing cells during the early stages of development in the central nervous system, peripheral nervous system and in myogenic and other tissues. Upon differentiation, nestin becomes downregulated and is replaced by tissue-specific intermediate filament proteins. During neuro- and gliogenesis, nestin is replaced by cell type-specific intermediate filaments, e.g. neurofilaments and glial fibrillary acidic protein (GFAP). Interestingly, nestin expression is reinduced in the adult during pathological situations, such as the formation of the glial scar after CNS injury and during regeneration of injured muscle tissue. Nestin is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: FLJ21841; intermediate filament protein; Nbla00170; NES; Nestin
Gene Aliases: AA166324; C78523; ESTM46; Nbla00170; NES
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.