|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:20-1:200|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20-1:200|
|Western Blot (WB)||Assay Dependent|
|Tested Species reactivity||Mouse, Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Nestin purified from embryonic rat spinal cord.|
|Contains||0.09% sodium azide|
|Storage conditions||4°C or -20°C if preferred|
This antibody does not react with human.
In Western blot, this antibody detects a ~200-220 kDa band in newborn rat or mouse cell extracts. For immunohistochemistry, we recommend perfusing tissues with 4% paraformaldehyde (pH 7.4) for light microscopy and with either 4% paraformaldehyde (pH 10.0) or 4% paraformaldehyde with 0.1% glutaraldehyde (pH 7.4) for EM. For Immunocytochemistry we recommend using cells fixed in 4% paraformaldehyde buffered with 50mM sodium borate (pH 9.5). For Western blot, it is recommended that samples should be boiled in 4 volumes of 125 mM Tris, pH 6.8, 10% 2-mercaptoethanol, 10% glycerol and 4.6% SDS. Membranes should be blocked with milk or BSA. 5% PAGE gels are suggested.
This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: FLJ21841; intermediate filament protein; Nbla00170; NES; Nestin
Gene Aliases: AA166324; C78523; ESTM46; Nes
UniProt ID: (Mouse) Q6P5H2
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