|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1:100|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to C-terminus of human nestin.|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is kidney tissue.
This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: FLJ21841; Nbla00170; NES; Nestin
Gene Aliases: Nbla00170; NES
UniProt ID: (Human) P48681
Entrez Gene ID: (Human) 10763
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