|Immunofluorescence (IF)||Assay dependent|
|Western Blot (WB)||1:1,000|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Human, Rat|
|Published species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Bacterially expressed coiled-coil domain of rat neurabin I.|
|Storage buffer||whole serum diluted in PBS|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA3-064 detects neurabin-1 from human and rat cells and tissues.
PA3-064 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~123 kDa protein representing neurabin-1 from rat brain extract. Immunofluorescence staining of neurabin-1 in in cultured rat hippocampal cells with PA3-064 results in diffuse cytoplasmic staining.
PA3-064 antigen is bacterially expressed coiled-coil domain of rat neurabin-1.
Neurabin I is a brain-specific protein which contains an F-actin binding domain, a PDZ domain, a transmembrane-protein-interacting-domain, and a coiled-coil region. As its structure suggests, neurabin I is a promiscuous protein binding to F-actin, protein phosphatase 1, TGN38, and p70 S6 kinase. Found exclusively in brain, this protein is highly concentrated in the synapse and has also been shown to be enriched in the lamellipodia of the growth cone during neuronal development. Neurabin I appears to be a bridging protein by targeting other proteins to the synapse or by linking membrane proteins to the actin cytoskeleton.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: actin-binding protein neurabin; neurabin 1; Neurabin-1; Neurabin-I; Neural tissue-specific F-actin-binding protein I; Neural Tissue-Specific F-Actin-Binding Protein-I; p180; PP1bp175; PPP1R9A; Protein phosphatase 1 regulatory subunit 9A; protein phosphatase 1, regulatory (inhibitor) subunit 9A; protein phosphatase 1, regulatory subunit 9A
Gene Aliases: KIAA1222; Neb1; Neurabin-I; NRB1; NRBI; PPP1R9A
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