|Immunocytochemistry (ICC)||1-2 µg/ml|
|Immunofluorescence (IF)||1-2 µg/ml|
|Immunohistochemistry (IHC)||5-10 ug/mL|
|Western Blot (WB)||1-3 µg/ml|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide corresponding to the N-terminal residues 27-41 of human neurofibromin.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA1-085 detects neurofibromin in human, rat, and mouse cells.
MA1-085 has been successfully used in Western blot, and immunohistochemistry procedures. By Western blot, this antibody detects a ~240-260 kDa protein.
The MA1-085 immunogen is a synthetic peptide corresponding to the N-terminal residues 27-41 of human neurofibromin.
Neurofibromin is a member of the GTPase-activating (GAP) class of proteins. These proteins enhance the GTPase activity of signaling G-Proteins like Ras. The NF1 gene is also classified as a tumor suppressor gene, and many studies have shown that mutations at the NF1 locus produce neurofibromas, rhabdomyosarcomas, leimyosarcomas, mental retardation and memory deficiency. Neurofibromin has also been associated with the intermediated filaments of the cytoskeleton. NF1 is expressed at its highest levels during periods of desmosome formation and mutations of NF1 produce variable cell sizes and morphologies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: neurofibromatosis 1; Neurofibromatosis type 1; Neurofibromatosis-related protein NF-1; Neurofibromin; NF1; NFNS; VRNF; WSS
Gene Aliases: AW494271; E030030H24Rik; Nf-1; NF1; NFNS; VRNF; WSS
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.