|Western Blot (WB)||1:10,000|
|Tested Species reactivity||Bovine, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Native NF-H purified from bovine spinal cord|
|Storage buffer||whole serum|
|Contains||10mM sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
PA1-10016 detects Neurofilament NF-H, primarily the hyperphosphorylated axonal form which yields a band at ~200kDa in Western blot applications.
Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (~70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 200 kDa neurofilament protein; neurofilament heavy polypeptide; neurofilament triplet H protein; NF 200 kD; NF-H; NFH; NHC
Gene Aliases: Nfh
Entrez Gene ID: (Rat) 24587
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