|ELISA (ELISA)||Assay dependent|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2-3 µg/mL|
|Immunofluorescence (IF)||2-3 µg/mL|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Western Blot (WB)||1-2 µg/mL|
|Miscellaneous PubMed (MISC)||See 3 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human Chicken|
|Host / Isotype||Mouse|
|Immunogen||Enzymatically dephosphorylated porcine neurofilaments.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA1-2010 detects the neurofilament, light chain in human, rat, and mouse samples.
MA1-2010 has been successfully used in western blot, immunohistochemistry, and immunofluorescence. By western blot this antibody detects a ~70 kDa protein representing the neurofilament, light chain in mouse brain tissue lysate. In immunohistochemistry procedures MA1-2010 recognizes the neurofilament, light chain in mouse brain tissue. In immunofluorescence procedures MA1-2010 recognizes the neurofilament, light chain in rat cerebral cortices.
The MA1-2010 immunogen is enzymatically dephosphorylated porcine neurofilaments.
Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-L is the light or low molecular weight microfilament subunit and runs on SDS-PAGE gels at approximately 70 kDa.
Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (~70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 68 kDa neurofilament protein; 70 kDa neurofilament protein; CMT1F; CMT2E; light molecular weight neurofilament protein; Neurofilament light polypeptide; neurofilament protein L; neurofilament protein, light chain; neurofilament subunit NF-L; Neurofilament triplet L pro; Neurofilament triplet L protein; neurofilament, light polypeptide 68kDa; NF-L; NFL; NLC; protein phosphatase 1, regulatory subunit 110
Gene Aliases: AI847934; CMT1F; CMT2E; NEFL; NF-L; NF68; NFL; PPP1R110
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