|Immunofluorescence (IF)||3-5 ug/ml|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:500|
|Immunohistochemistry (PFA fixed) (IHC (PFA))||Assay Dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:100-1:1000|
|Immunocytochemistry (ICC)||See 1 publications below|
|Tested Species reactivity||Human, Rat|
|Published species reactivity||Human|
|Host / Isotype||Mouse|
|Immunogen||Synthetic peptide corresponding to a C-terminal fragment of rat neurofilament.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA1-2011 detects the neurofilament, medium chain in human and rat samples.
MA1-2011 has been successfully used in Western blot, immunofluorescent, and immunoprecipitation procedures. By Western blot this antibody detects a ~160 kDa protein representing the neurofilament, medium chain in HEK 293 cells, rat spinal cord, and human frontal cortex. In immunofluorecence procedures MA1-2011 recognizes the neurofilament, medium chain in human neurons.
The MA1-2011 immunogen is a synthetic peptide corresponding to a C-terminal fragment of rat neurofilament.
Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (~70, 160, and 200 kDa) occur in both the central and
peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 160 kD neurofilament protein; 160 kDa neurofilament protein; NEF3; Neurofilament 3; neurofilament 3, medium; Neurofilament medium polypeptide; Neurofilament protein, middle polypeptide; Neurofilament triplet M protein; neurofilament, medium polypeptide 150kDa; neurofilament-3 (150 kD medium); NF-M; NFM; NMC
Gene Aliases: NEF3; NEFM; NF-M; NFM
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