|Western Blot (WB)||1:20,000|
|Tested Species reactivity||Bovine, Chicken, Horse, Human, Mouse, Pig, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fusion protein containing the extreme C-terminal segment of rat NF-M|
|Storage buffer||whole serum|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
In Western blot, this antibody detects bands at ~145kDa in crude homogenates of rat cerebellum homogenate. Human and bovine run slower at ~160kDa. A minor band at1~110kDa is also frequently seen, which appears to be an in-vivo proteolytic fragment of NF-M.
Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (~70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 160 kD neurofilament protein; 160 kDa neurofilament protein; major neuronal intermediate filament subunit; NEF3; Neurofilament 3; neurofilament 3 (150kDa medium); neurofilament 3, medium; neurofilament M subunit; Neurofilament medium polypeptide; neurofilament protein M; Neurofilament protein, middle polypeptide; Neurofilament triplet M protein; neurofilament, medium polypeptide 150kDa; neurofilament-3 (150 kD medium); neurofilament-M; NF-M; NF-M c-terminus; NF-M protein; NFM; NMC
Gene Aliases: BOS_9093; NEF3; NEFM; NF-M; NF160; NF165; NFM
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