|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:300|
|Western Blot (WB)||1:100-1:2000|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Mouse|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Purified full length native human NSE protein.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Antigen retrieval is not required but may optimize staining of paraffin tissue sections.
Suggested positive control: antigen standard for ENO2 (transient overexpression lysate).
Enolase is a glycolytic enzyme catalyzing the reaction pathway between 2 phospho glycerate and phosphoenol pyruvate. In mammals, enolase molecules are dimers composed of three distinct subunits (alpha, beta and gamma). The alpha subunit is expressed in most tissues and the beta subunit only in muscle. The gamma subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. NSE (neuron specific enolase) is found in elevated concentrations in plasma and certain neoplasias. These include pediatric neuroblastoma and small cell lung cancer. Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 2-phospho-D-glycerate hydro-lyase; 2-phospho-D-glycerate hydrolyase; Eno; ENOG; Enolase 2; enolase 2 (gamma, neuronal); epididymis secretory protein Li 279; Gamma-enolase; glycerate hydrolyase; Neural Enolase; Neuron Specific Enolase; neuron specific gamma enolase; Neuron-specific enolase; Neuronal Enolase; neuronal enriched enolase; neurone-specific enolase; NSE
Gene Aliases: ENO2; HEL-S-279; NSE
UniProt ID: (Human) P09104
Entrez Gene ID: (Human) 2026
Molecular Function: lyase
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