|ELISA (ELISA)||Assay Dependent|
|Immunoelectrophoresis (IE)||Assay Dependent|
|Tested Species reactivity||Human|
|Host / Isotype||Sheep / IgG|
|Immunogen||Neutrophil elastase from human sputum.|
|Storage buffer||0.01M HEPES, pH 7.4, with 0.15M NaCl, 50% glycerol|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-74134 detects Neutrophil Elastase from human samples.
PA1-74134 has been successfully used in ELISA and Immunoelectrophoresis applications.
The PA1-74134 immunogen is Nuetrophil elastase from human sputum.
Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins. The product of this gene hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the proteiNULLs release from activated neutrophils. The enzyme may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. This protein degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is clustered with other serine protease gene family members, azurocidin 1 and proteinase 3 genes, at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: bone marrow serine protease; elastase 2, neutrophil; elastase-2; granulocyte-derived elastase; human leukocyte elastase; leukocyte elastase; medullasin; neutrophil elastase; PMN elastase; polymorphonuclear elastase
Gene Aliases: ELA2; GE; HLE; HNE; NE; PMN-E; SCN1
UniProt ID: (Human) P08246
Entrez Gene ID: (Human) 1991
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