|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Immunohistochemistry (IHC)||See 1 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide near the C-terminus of mouse iNOS protein.|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is lung tissue.
Inducible NOS (iNOS) or macrophage NOS (mNOS) is calcium/calmodulin independent and is expressed in activated macrophages and stimulated glial cells. NOS oxidizes a guanidine nitrogen of arginine, releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation, neurotransmission, anti-tumor and anti-pathogenic activities.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: HEP-NOS; Hepatocyte NOS; hepatocytes; inducible; Inducible NO synthase; Inducible NOS; INOS; macrophage; nitric oxide synthase; nitric oxide synthase 2, inducible; nitric oxide synthase 2A (inducible, hepatocytes); nitric oxide synthase 2A inducible; Nitric oxide synthase, inducible; nitric oxide synthase, macrophage; NOS; NOS type II; NOS, type II; NOS2A; Peptidyl-cysteine S-nitrosylase NOS2; type II
Gene Aliases: HEP-NOS; INOS; NOS; NOS2; NOS2A
UniProt ID: (Human) P35228
Entrez Gene ID: (Human) 4843
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.