|Immunohistochemistry (Paraffin) (IHC (P))||1:3000|
|Western Blot (WB)||1:1000-1:5000|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated Cys-VRSIAKDVALACKTSETVPRPA|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
IHC (P) analysis shows positive staining of NOP in human enteric ganglion.
WB analysis shows NOP in glycoprotein-enriched fractions from mock transfected or NOP overexpressing 293 cells.
While NOP has a theoretical MW of ~ 41kD, the observed band in a WB runs at ~72kD due to enrichment of the glycosylated receptor.
The G-protein-coupled receptor (GPCR) superfamily is comprised of an estimated 600-1,000 members and is the largest known class of molecular targets with proven therapeutic value. The nociceptin receptor is a G-protein coupled opioid receptor that functions as receptor for the endogenous neuropeptide nociceptin. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling via G proteins mediates inhibition of adenylate cyclase activity and calcium channel activity. Arrestins modulate signaling via G proteins and mediate the activation of alternative signaling pathways that lead to the activation of MAP kinases. Plays a role in modulating nociception and the perception of pain. Plays a role in the regulation of locomotor activity by the neuropeptide nociceptin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Kappa-Type 3 Opioid Receptor; Kappa3-Related Opioid Receptor; KOR-3; LC132 Receptor-Like; Nociceptin Receptor; nociceptin/orphanin FQ receptor; NOCIR; NOP; NOPR; OOR; Opiate Receptor-Like 1; OPPRL1; ORL1; Orphanin FQ Receptor
Gene Aliases: KOR-3; NOCIR; NOP; NOPr; OOR; OPRL1; ORL1
UniProt ID: (Human) P41146
Entrez Gene ID: (Human) 4987
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.