|ELISA (ELISA)||Assay dependent|
|Flow Cytometry (Flow)||Assay dependent|
|Inhibition Assays (IA)||Assay Dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||10 µg/ml|
|Flow Cytometry (Flow)||See 2 publications below|
|ELISA (ELISA)||See 1 publications below|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Many|
|Published species reactivity||Pig , Hamster , Fish Human|
|Host / Isotype||Mouse / IgM|
|Immunogen||Purified NCC cells from catfish.|
|Storage buffer||1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA1-760 detects nonspecific cytotoxic cells (NCC) from catfish and tilapia tissues and also detects natural killer/lymphokine activated killer (NK/LAK) cells from rat, bovine, equine, canine and porcine tissues. MA1-760 has been successfully used in Western blot, cytotoxic inhibition studies with NCC/NK/LAK cells, FACS, immunoprecipitation, panning, and ELISA procedures. By Western blot, this antibody detects a 32 kDa protein representing nonspecific cytotoxic cell receptor protein. In cytotoxicity assays, this antibody is used under saturating conditions to inhibit target cell conjugation. The MA1-760 immunogen is purified NCC cells from catfish.
Non-specific cytotoxic cells (NCCs) in teleosts and their evolutionary homologue are a subpopulation of lymphocytes with properties that distinguish them from either B- or T-cells. One such property is that NCC/natural killer (NK)/lymphokine activated killer (LAK) cells express spontaneous, non-major histocompatibility complex restricted cytotoxic activity. NCC and LAK lyse a variety of transformed murine and human B-cell, T-cell and myeloid targets. A 32 kDa membrane protein [non-specific cytotoxic cell receptor protein (NCCRP-1)] expressed by NCC and certain mammalian NK/LAK cells mediates this cytotoxicity. NCCRP-1 is evolutionarily conserved and is found in species ranging from marine and freshwater teleosts to higher mammals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Natural Killer Cell; NCC/NK
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