|Immunoprecipitation (IP)||2-4 µl/lysate|
|Western Blot (WB)||1:10,000-1:25,000|
|Tested Species reactivity||Yeast|
|Host / Isotype||Mouse / IgG3|
|Immunogen||Yeast nuclear preparation.|
|Storage buffer||tissue culture supernatant|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
MA1-10025 detects Yeast NOP-1 from S. cerevisiae samples and does not cross-react with fibrillarin in other species.
For chemiluminescent Western blot using yeast samples, use a dilution of 1:10,000 for cell lysates and 1:25,000 for nuclear fractions. For non-ECL detection methods, use a dilution of 1:1000-1:5000.
Nop1p is the yeast homologue of human fibrillarin, a component of the nucleolar small nuclear ribonucleoprotein (snRNP) particle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Histone-glutamine methyltransferase; LOT3; Nop1p; Nucleolar protein 1; rRNA 2'-O-methyltransferase fibrillarin; U3 small nucleolar RNA-associated protein NOP1; YDL014W
Gene Aliases: D2870; LOT3; NOP1; YDL014W
UniProt ID: (Baker's yeast) P15646
Entrez Gene ID: (Baker's yeast) 851548
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