|Immunohistochemistry (Paraffin) (IHC (P))||1:200|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from C-terminus of human notch-1|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is breast or ovarian carcinoma.
The 280-330kDa Notch proteins have been found to be overexpressed or rearranged in human tumors. Ligands for Notch include Jagged, Jagged-2 and Delta. Jagged can activate Notch and prevent myoblast differentiation by inhibiting the expression of muscle regulatory and structural genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: hN1; Neurogenic locus notch homolog protein 1; Notch 1; Notch homolog 1; Notch homolog 1, translocation-associated; TAN1; translocation-associated; Translocation-associated notch protein TAN-1
Gene Aliases: AOS5; AOVD1; hN1; NOTCH1; TAN1
UniProt ID: (Human) P46531
Entrez Gene ID: (Human) 4851
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