|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:1,000|
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
|Immunocytochemistry (ICC)||See 4 publications below|
|Western Blot (WB)||See 3 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
|Tested Species reactivity||Amphibian, Rat, Yeast|
|Published species reactivity||Yeast , Rat , Mouse , Human|
|Host / Isotype||Mouse / IgM|
|Immunogen||Pore complex-lamina fraction purified from rat liver nuclear envelopes.|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA1-071 detects nuclear pore-O-linked glycoprotein from rat, amphibian and yeast.
MA1-071 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects up to eight different proteins from the nuclear pore complex (NPC) of approximately 210, 180, 145, 100, 63, 58, 54 and 45 kDa. Immunofluorescence staining of NPC O-linked glycoproteins with MA1-071 results in exclusive labeling of the NPC proteins on a wide variety of mammalian cells as well as S. cerevisiae and Xenopus. Labeling occurs exclusively at the NPC with most of the labeling at the cytoplasmic and nucleoplasmic margins.
Microinjected MA1-071 inhibits both protein import and RNA export in Xenopus oocytes.
The MA1-071 immunogen is pore complex-lamina fraction purified from rat liver nuclear envelopes.
Diffusion of metabolites and small non-nuclear molecules as well as active, mediated import of protein and export of protein and RNA through the nuclear envelope occurs through nuclear pore complexes or NPC and quote;s. NPC and quote;s contain up to 100 different polypeptides which have a combined mass of about 125 megadaltons. The channel available for passive transport through the NPC is about 9-10 nm in diameter while carrier mediated changes in the NPC result in a ~25 nm channel used for larger, actively transported molecules. Of the 100 polypeptides, at least 8 of these are O-linked N-acetylglycosamine-modified in mammalian cells. All of the mammalian O-linked glycoproteins contain multiple copies of phenylalanine, glycine dipeptide repeats dispersed throughout part of their sequence. Studies indicate that the NPC O-linked glycoproteins have a direct role in nuclear protein import.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 62 kDa nucleoporin; Np62; nuclear pore glycoprotein 62; Nuclear pore glycoprotein p62; Nucleoporin Nup62
Gene Aliases: Np62; Nup62
UniProt ID: (Rat) P17955
Entrez Gene ID: (Rat) 65274
Molecular Function: transporter
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