|ChIP assay (ChIP)||Assay-Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Western Blot (WB)||1:4000|
|Tested Species reactivity||Human, Mouse, Plant, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Fusion protein containing amino acids 284-709 of human nucleolin.|
|Storage buffer||whole serum|
|Contains||0.1% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
Heat mediated antigen retrieval is recommended for IHC on paraffin embedded tissues.
Suggested positive control: PD31 murine pre-B cells for immunofluorescence and crude PD31 nuclear extracts for Western Blot.
Nucleolin is an abundant, 106 kDa nucleolar phosphoprotein. It is the major protein in actively dividing cells, whereas the degraded forms are relatively abundant in non-dividing cells. Stability of the nucleolin protein is cell proliferation-dependent. It plays a role in rDNA transcription, organization, and rRNA processing. It may alter DNA organization in response to cell cycle controls. It is also a good mammalian nucleolus marker.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: nucl; Nucleolin; nucleolin-like protein; Protein C23
Gene Aliases: B530004O11Rik; C23; D0Nds28; D1Nds28; NCL; Nuc; Nucl
Molecular Function: ribosomal protein
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