|Immunofluorescence (IF)||1 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||1:400|
|Immunohistochemistry (Paraffin) (IHC (P))||1 µg/ml|
|Western Blot (WB)||1 µg/ml|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Nucleolin.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
Heat mediated antigen retrieval recommended prior to tissue staining.
Store antibody at 4°C for 1-2 weeks. For long-term storage, store at -20°C.
Nucleolin , a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: C23; FLJ45706; FLJ59041; Nucleolin; nucleolin-like protein; Protein C23
Gene Aliases: B530004O11Rik; C23; D0Nds28; D1Nds28; NCL; Nuc; Nucl
Molecular Function: ribosomal protein
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