|ChIP assay (ChIP)||Assay Dependent|
|Dot blot (DB)||1/800|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:1,000|
|Western Blot (WB)||See 61 publications below|
|Immunoprecipitation (IP)||See 8 publications below|
|Miscellaneous PubMed (MISC)||See 3 publications below|
|ChIP assay (ChIP)||See 2 publications below|
|Immunohistochemistry (IHC)||See 5 publications below|
|Immunocytochemistry (ICC)||See 3 publications below|
|ELISA (ELISA)||See 1 publications below|
|Tested Species reactivity||All|
|Published species reactivity||Rat , Fruit fly , Non-human primate , Amphibian , Hamster , Fish , Human , Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Pore complex-lamina fraction purified from rat liver nuclear envelopes.|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA1-072 detects nuclear pore complex (NPC), cytoplasmic and intranuclear O-linked glycoproteins from human, mouse, virus and rat tissues.
MA1-072 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody recognizes up to eight different proteins from the NPC of approximately 210, 180, 145, 100, 63, 58, 54 and 45 kDa as well as other O-linked glycoproteins outside of the NPC. Immunofluorescence staining of the NPC with MA1-072 primarily labels NPC O-linked glycoproteins and has been successfully used on a wide variety of mammalian cells. Labeling occurs at the NPC, with most of the labeling at the cytoplasmic and/or nucleoplasmic margins, as well as within the nucleus.
The MA1-072 immunogen is pore complex-lamina fraction purified from rat liver nuclear envelopes.
Diffusion of metabolites and small non-nuclear molecules as well as active, mediated import of protein and export of protein and RNA through the nuclear envelope occurs through nuclear pore complexes or NPC and quote;s. NPC and quote;s contain up to 100 different polypeptides which have a combined mass of about 125 megadaltons. The channel available for passive transport through the NPC is about 9-10 nm in diameter while carrier mediated changes in the NPC result in a ~25 nm channel used for larger, actively transported molecules. Of the 100 polypeptides, at least 8 of these are O-linked N-acetylglycosamine-modified in mammalian cells. All of the mammalian O-linked glycoproteins contain multiple copies of phenylalanine, glycine dipeptide repeats dispersed throughout part of their sequence. Studies indicate that the NPC O-linked glycoproteins have a direct role in nuclear protein import.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: GlcNAc; O-GlcNAc transferase p110 subunit; O-GlcNAc transferase subunit p110; O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase); O-linked N-acetylglucosamine transferase 110 kDa subunit; OGT; UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit; UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase; uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyl transferase
Gene Aliases: HINCUT-1; HRNT1; O-GLCNAC; OGT
UniProt ID: (Human) O15294
Entrez Gene ID: (Human) 8473
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