|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the C-terminus of human Oct-1|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is tonsil tissue.
The POU dimmer from the OCT-1 gene formed on the palindromic OCT factor recognition element, or PORE (ATTTGAAATGCAAAT), could recruit the transcriptional coactivator OBF1. OCT-1 encodes one protein, NF-A1, which is found in nuclear extracts from all cell types and thus is not specific to lymphoid cells as is the protein NF-A2, which is encoded by OCT-2. The OCT-1 and 2 map to human chromosomes 1q22-q23 and 19, respectively.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: class 2; NF-A1; Oct-1; oct-1B; OCT1; Octamer-binding protein 1; Octamer-binding transcription factor 1; Octamer-binding transcription factor-1; OTF-1; OTF1; POU domain; POU domain, class 2, transcription factor 1; transcription factor 1
Gene Aliases: oct-1B; OCT1; OTF1; POU2F1
UniProt ID: (Human) P14859
Entrez Gene ID: (Human) 5451
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