|Immunohistochemistry (IHC)||Assay Dependent|
|Neutralization (Neu)||Assay Dependent|
|Western Blot (WB)||5-10 µg/ml|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein corresponding to human OGR1 (GPR68) protein.|
|Purification||Ammonium sulfate precipitation|
|Contains||0.08% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
Western blot should be visualized using a high sensitivity secondary/substrate system (i.e SuperSignal West Femto Maximum Sensitivity Substrate). A suggested positive control for this product is OGR1 transfected cell lysate.
OGR1 (originally identified as GPR68) is a high-affinity receptor for sphingosylphosphorylcholine (SPC). SPC is a member of a class of bioactive lipids which regulate cellular functions. These functions include cell proliferation and growth inhibition, smooth muscle contration and wound healing. SPC also stimulated signalling pathways that induce tyrosine phosphorylation, activate MAP kinases and protein kinase C, modify ion channel activity and increase intracellular Ca2+ concentration. OGR1 is expressed in placenta, lung, liver, spleen, testis, small intestine and peripheral blood leukocytes. It is not found in thymus, ovary, colon, skeletal muscle, kidney or pancreas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: G Protein-Coupled Receptor OGR1/GPR68; G-protein coupled receptor 68; GPR12A; OGR-1; ovarian cancer G protein-coupled receptor, 1; Ovarian cancer G-protein coupled receptor 1; Sphingosylphosphorylcholine receptor
Gene Aliases: GPR12A; GPR68; OGR1
UniProt ID: (Human) Q15743
Entrez Gene ID: (Human) 8111
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