|Flow Cytometry (Flow)||Assay-Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:10-1:500|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:500|
|Western Blot (WB)||1 µg/ml|
|Tested Species reactivity||Human, Rat|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||A peptide corresponding to the amino acids 20-37 TQETFPPKYLHYDEETSH of human OPG was used as immunogen for this antibody.|
|Contains||0.05% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
Suggested positive control: Daudi.
Osteoprotegerin (OPG) is a secretory glycoprotein belonging to TNF receptor (TNFR) superfamily (1). As the name implies, it protects bone. Unlike other TNFRs it lacks a transmembrane domain and lacks any apparent cell associated signals. OPG consists of 401 amino acid with molecular mass of approximately 55 kD as a monomer and 110 kD as a disulfide-linked dimer. High levels of OPG mRNA has been detected in lung, heart, kidney, and placenta1. Recombinant OPG blocks osteoclastogenesis in vitro and increases bone density in vivo. Targeted deletion of OPG in mice results in severe early-onset of osteoporosity. These mice also exhibit increase in vascular calcification in the aorta and renal arteries suggesting that it may also play a role in degenerative arterial disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: OCIF; OPG; Osteoclastogenesis inhibitory factor; Osteoprotegerin; TR1; Tumor necrosis factor receptor superfamily member 11B; tumor necrosis factor receptor superfamily, member 11b
Gene Aliases: OCIF; OPG; PDB5; TNFRSF11B; TR1
UniProt ID: (Human) O00300
Entrez Gene ID: (Human) 4982
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.