|Immunocytochemistry (ICC)||5-15 µg/ml|
|Immunofluorescence (IF)||5-15 µg/ml|
|Western Blot (WB)||0.1 µg/ml|
|Tested Species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Recombinant human Otx2, corresponding to Met1-Leu289|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with trehalose|
|Storage conditions||-20°C or -80°C if preferred|
In direct ELISAs, approximately 10% cross-reactivity with recombinant human OTX1 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
OTX2 is a 289 amino acid containing protein encoding a member of the bicoid sub-family of homeodomain-containing transcription factors. It has two known alternative splice variants with distinct isoforms though presence of other isoforms cannot be excluded. The protein is an early marker of endodermal differentiation of embryonic stem cells and may play a role in the development of the brain and the sensory organs. The protein may bind to BCD target sequence (BTS): 5'-TCTAATCCC-3'. Defects in OTX2 are the cause of microphthalmia syndromic type 5 (MCOPS5).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CPHD6; Homeobox protein OTX2; MCOPS5; Orthodenticle homolog 2
Gene Aliases: CPHD6; MCOPS5; OTX2
UniProt ID: (Human) P32243
Entrez Gene ID: (Human) 5015
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