|Immunohistochemistry (IHC)||1:300 - 1:2000|
|Western Blot (WB)||1:300 - 1:2000|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit|
|Immunogen||A synthetic peptide from aa region 420-470 of human melanopsin conjugated to an immunogenic carrier protein was used as the antigen|
|Storage buffer||whole serum|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
Glycerol (1:1) may be added for additional stability.
Reconstitute in 100 uL of sterile water.
Opsins are members of the guanine nucleotide-binding protein (G protein)-coupled receptor superfamily. This gene encodes a photoreceptive opsin protein that is expressed within the ganglion and amacrine cell layers of the retina. In mouse, retinal ganglion cell axons expressing this gene projected to the suprachiasmatic nucleus and other brain nuclei involved in circadian photoentrainment. In mouse, this protein is coupled to a transient receptor potential (TRP) ion channel through a G protein signaling pathway and produces a physiologic light response via membrane depolarization and increased intracellular calcium. The protein functions as a sensory photopigment and may also have photoisomerase activity. Experiments with knockout mice indicate that this gene attenuates, but does not abolish, photoentrainment. Alternative splicing results in multiple transcript variants encoding different isoforms. Photoreceptor required for regulation of circadian rhythm. Contributes to pupillar reflex and other non-image forming responses to light. May be able to isomerize covalently bound all-trans retinal back to 11-cis retinal. Found in soma, dendrites and proximal part of axons of certain retinal ganglion cells. Expression is restricted within the ganglion and amacrine cell layers of the retina.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Melanopsin; MGC142118; OPN 4; OPN4; Opsin-4
Gene Aliases: MOP; OPN4
UniProt ID: (Human) Q9UHM6
Entrez Gene ID: (Human) 94233
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