|Immunohistochemistry (Paraffin) (IHC (P))||Neat|
|Tested Species reactivity||Dog, Human, Pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide derived from internal domain of human Osteopontin.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
PA1-21083 detects Osteopontin prediluted in human samples. Expected to cross react with canine and porcine due to sequence homology.
PA1-21083 has been successfully used in Immunohistochemistry (Paraffin) procedures. Staining of formalin fixed tissue sections requires boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. A suggested positive control for this product is breast carcinoma.
PA1-21083 immunogen corresponds to A synthetic peptide derived from internal domain of human Osteopontin.
Osteopontin is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein that interacts with integrins and CD44 as major receptors. OPN is multifunctional, with activities in cell migration, cell survival, inhibition of calcification, regulation of immune cell function, and control of tumor cell phenotype. Targeting of the gene encoding Osteopontin, has revealed that while it is not necessary for normal embryonic development, fertility, and health under pathogen-free conditions, loss of the protein has significant consequences in several models of injury/disease as diverse as renal injury, viral and bacterial infection, bone remodeling, and tumor growth. The fact that no other proteins seem to share a redundant activity with Osteopontin under these conditions suggests that it has a unique functional role during tissue injury and stress.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Bone sialoprotein 1; early T-lymphocyte activation 1; Nephropontin; Osteopontin; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; Secreted phosphoprotein 1; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); secreted phosphoprotein 1 variant 6; SPP-1; SPP1/CALPHA1 fusion; Urinary stone protein; Uropontin
Gene Aliases: BNSP; BSPI; ETA-1; OPN; PSEC0156; SPP1
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