|Western Blot (WB)||7.2 ug/ml|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Bovine, Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / Ig Mix|
|Storage buffer||HEPES buffered saline|
|Contains||0.02% sodium azide|
|Storage conditions||4° C, do not freeze|
This product is a cocktail of multiple OxPhos Mouse anti Human antibodies.
C-I-20 (ND6) (clone) 20E9DH10C12 - Bovine Complex I
This is an optimized premixed cocktail suitable for Western Blotting analysis of the relative levels of the 5 OxPhos complexes in mitochondrial preparations from human sources. Human heart mitochondria (50 µg) is also included as a Western Blotting control. These clones were chosen because they are against a subunit that is labile when its complex is not assembled. Moreover, the combination is readily resolved in SDS-PAGE when the appropriate gel conditions are used.
C-II-30 (FeS) (clone) 21A11AE7 - Bovine Heart Complex II
C-III-Core 2 (clone) 13G12AF12BB11 - Bovine Heart Complex II, III
C-IV-II (clone) 12C4F12 -Human Complex IV, subunit II
C-V-alpha (clone) 15H4C4 -Bovine Complex V
Altered levels of assembly can arise from mutations in individual subunits, mutations in assembly factors for the complex(es), mtDNA depletion or as a result of physiological and or pathological changes e.g. hormone treatment, exercise, diet or oxidative stress.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Oxidative Phosphorylation; OxPhos Complex
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