|Flow Cytometry (Flow)||5-10 µg for 1x10^6 cells|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||5-10 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1-10 µg/ml|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1-10 µg/ml|
|Western Blot (WB)||See 4 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Tested Species reactivity||Hamster, Human, Mouse, Rat|
|Published species reactivity||Dog , Rat Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||SDS-solubilized plasma membranes of a multidrug resistant Chinese hamster ovary (CHO) cell line and a human cell line.|
|Storage buffer||PBS with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA1-26528 detects P Glycoprotein from human, hamster, mouse and rat samples. MA1-26528 is expected to cross react with a wide range of mammals due to sequence homology.
MA1-26528 has been successfully used in immunoprecipitation, immunocytochemistry, immunohistochemistry, flow cytometry, and Western blot procedures.
The MA1-26528 immunogen is SDS-solubilized plasma membranes of a multidrug resistant Chinese hamster ovary (CHO) cell line and a human cell line. This antibody recognizes a highly conserved internal sequence (VQEALD and VQAALD) corresponding to the C- and N-terminal regions, respectively, from both MDR1 and MDR3 isoforms of P Glycoprotein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ABC20; ATP-binding cassette sub-family B member 1; ATP-binding cassette sub-family B member 1A; ATP-binding cassette, sub-family B (MDR/TAP), member 1; ATP-binding cassette, sub-family B (MDR/TAP), member 1A; ATP-binding cassette, sub-family B (MDR/TAP), member 4; ATP-binding cassette, subfamily B (MDR/TAP), member 1A; ATP-binding cassette, subfamily B, member 1A; CD243; CLCS; colchicin sensitivity; doxorubicin resistance; ecotropic viral integration site 32; GP170; MDR1; MDR1A; multi-drug resistance 3; Multidrug resistance protein 1; Multidrug resistance protein 1A; Multidrug resistance protein 3; multiple drug resistant 1a; P glycoprotein 3; P-glycoprotein 1; P-glycoprotein 3; P-GP; PGY1
Gene Aliases: ABC20; ABCB1; Abcb1a; Abcb4; CD243; CLCS; Evi32; GP170; mdr-3; MDR1; Mdr1a; Mdr3; P-GP; Pgp; PGP1; Pgy-3; PGY1; Pgy3
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