|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to aa 525-545 of rat alpha PAK|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is breast carcinoma.
Alpha PAK p68, beta PAK p65 and gamma PAK p62 are three isoforms of serine/threonine kinases. These three isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Gamma-PAK; p21 (CDKN1A)-activated kinase 2; p21 CDKN1A-activated kinase 2; p21 protein (Cdc42/Rac)-activated kinase 2; p21-activated kinase 2; p58; PAK-2; PAK65; PAKgamma; S6/H4 kinase; Serine/threonine-protein kinase PAK 2
Gene Aliases: PAK2; PAK65; PAKgamma
UniProt ID: (Human) Q13177
Entrez Gene ID: (Human) 5062
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