|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
|Western Blot (WB)||1:25|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from C-terminus of human DJ-1 protein|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
This antibody is predicted to react with bovine, chicken, canine, mouse, porcine and rat based on sequence homology.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is normal breast tissue.
Four chromosomal loci (PARK2, PARK6, PARK7, and PARK9) associated with autosomal recessive, early onset parkinsonism are known. Positional cloning within the refined PARK7 critical region recently identified mutations in the DJ-1 gene in PARK7-linked families. Mutations in the PARK7/DJ-1 gene cause autosomal-recessive Parkinsons disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DJ-1; epididymis secretory sperm binding protein Li 67p; Oncogene DJ1; Parkinson disease (autosomal recessive, early onset) 7; Parkinson disease protein 7; parkinson protein 7; Parkinsonism-associated deglycase; Protein deglycase DJ-1; Protein DJ-1
Gene Aliases: DJ-1; DJ1; HEL-S-67p; PARK7
UniProt ID: (Human) Q99497
Entrez Gene ID: (Human) 11315
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