|Immunofluorescence (IF)||1:20 - 1:200|
|Immunoprecipitation (IP)||3 µg|
|Western Blot (WB)||1:1000 - 1:2000|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Tested Species reactivity||Human, Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Purified internal fragment of human recombinant PAX7 expressed in E. coli.|
|Storage buffer||PBS with 30% glycerol, 1mg/ml BSA|
|Contains||0.05% sodium azide|
Western blot analysis of PA1-117 detects a prominent ~54 kDa protein in human HeLa, MCF7, rhabdomyosarcoma and skeletal muscle satellite cells and a weaker ~57 kDa protein in HeLa, rhabdomyosarcoma and skeletal muscle satellite cells. The ~57 kDa band seems to be enriched in immunoprecipitations from HeLa lysate, suggesting a bias for the larger isoform in ths application. Weak unknown bands of lower molecular weight and at ~110-130 kDa are also detected. Specificity of the antibody was also confirmed in Hela cells overexpressing full length PAX7.
PAX7 is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. Pax7 is expressed in muscle satellite cells. The satellite cells are required for tissue repair and regeneration following muscle injury, and Pax7 expression is required to maintain survival and proliferation of postnatal satellite cells in adults. The chromosomal translocation t(1;13)(p36;q14) with FOXO1 in alveolar rhabdomyosarcomas, results in PAX7-FOXO1 fusion protein which acts as a transcriptional activator. Alternative splicing yields 2 isoforms, PAX7-A (57 kDa) and PAX7-B (54 kDa) (PMID: 15527970).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: HUP1; paired box 7; paired box gene 7; paired box homeotic gene 7; Paired box protein Pax-7; paired box transcription factor PAX7; paired domain gene 7; PAX7 transcriptional factor; PAX7A; PAX7B; RMS2
Gene Aliases: HUP1; Pax-7; PAX7; PAX7B; RMS2
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