|Immunoprecipitation (IP)||5-10 µg|
|Western Blot (WB)||1:500|
|Tested Species reactivity||Dog, Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide, conjugated to KLH, corresponding to residues E(817) K F F F S K I K E A G L I D K(832) of human PCAF, with an N-terminal added cyst|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||15mM sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
PA1-25467 detects PCAF from human and canine samples and is expected to cross react with mouse and chicken due to sequence homology.
PA1-25467 has been successfully used in Western blot and immunoprecipitation procedures.
The PA1-25467 immunogen is a synthetic peptide, conjugated to KLH, corresponding to residues E(817) K F F F S K I K E A G L I D K(832) of human PCAF, with an N-terminal added cysteine.
Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.
CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CAF; CREBBP-associated factor; Histone acetylase PCAF; Histone acetyltransferase KAT2B; Histone acetyltransferase PCAF; K(lysine) acetyltransferase 2B; Lysine acetyltransferase 2B; P; P/CAF; P300/CBP-associated factor; PCAF
Gene Aliases: A930006P13Rik; AI461839; AW536563; CAF; KAT2B; P/CAF; PCAF
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