|Flow Cytometry (Flow)||3-5 µg/million cells|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Western Blot (WB)||1:1000|
|Immunohistochemistry (Paraffin) (IHC (P))||See 3 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Immunohistochemistry (Paraffin, Frozen) (IHC (P, F))||See 2 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Human, Insect, Mouse, Non-human primate, Yeast|
|Published species reactivity||Sheep , Mouse , Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Rat recombinant PCNA polypeptides.|
|Storage buffer||PBS, pH 7.4, with 50% glycerol, 1% BSA|
|Contains||0.1% sodium azide|
|Storage conditions||4° C|
PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. It is found in the nucleus and is a cofactor of DNA polymerase delta. It acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Cyclin; DNA polymerase delta auxiliary protein; PCNA; Proliferating cell nuclear antigen
Gene Aliases: ATLD2; PCNA
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