|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Immunoprecipitation (IP)||5 µg|
|Western Blot (WB)||1:500-1:3000|
|Immunohistochemistry (Paraffin) (IHC (P))||See 2 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 253 of Human PCNA|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Contains||0.025% ProClin 300|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA5-27214 targets PCNA in IF, IHC (P), and WB applications and shows reactivity with Human, mouse, and Rat samples.
The PA5-27214 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 253 of Human PCNA.
The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Cyclin; DNA polymerase delta auxiliary protein; PCNA; Proliferating cell nuclear antigen
Gene Aliases: ATLD2; PCNA; Pcna/cyclin; PCNAR
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