|Flow Cytometry (Flow)||1:200-1:1000|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200-1:400|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1:50-1:1000|
|Immunohistochemistry (IHC)||See 94 publications below|
|Immunocytochemistry (ICC)||See 8 publications below|
|Western Blot (WB)||See 29 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
|Immunoprecipitation (IP)||See 2 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Tested Species reactivity||Dog, Human, Non-human primate|
|Published species reactivity||Yeast , Rat , Pig , Non-human primate , Amphibian , Zebrafish , Cat , Mouse , Human , , Xenopus , Rhesus monkey Guinea pig Dog|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||Recombinant rat PCNA protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
Methanol fixation is required for immunofluorescence and flow cytometry staining. To decrease nonspecific staining for both applications, using a Biotin/Streptavidin blocker is recommended.
By WB, MA5-11355 detects a predominant band between 30-35kD, and fainter nonspecific bands at ~72kD and 130kD.
Expression of proliferating cell nuclear antigen (PCNA) or cyclin or polymerase delta auxiliary protein is elevated in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. Its level correlates directly with rates of cellular proliferation and DNA synthesis. PCNA/cyclin may act as an auxiliary protein of DNA polymerase-delta to play a fundamental role in the initiation of cell proliferation
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Cyclin; DNA polymerase delta auxiliary protein; PCNA; Proliferating cell nuclear antigen
Gene Aliases: ATLD2; PCNA
UniProt ID: (Human) P12004
Entrez Gene ID: (Human) 5111
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