|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from near C-terminus of human PDGFR-beta|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
This antibody is predicted to react with chicken, canine, amphibian, mouse, porcine, rabbit and rat based on sequence homology.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is prostate, skin or ovarian carcinoma.
PDGFR (Platelet Derived Growth Factor Receptor) exhibits two different isoforms, alpha and beta coded by two different genes. PDGF A receptors are expressed in oligodendrocyte progenitor cells and PDGF B receptors are expressed on neurons. This antibody is specific for PDGFR, alpha and does not react with PDGFR, beta.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Beta platelet-derived growth factor receptor; Beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; CD140b; JTK12; PDGF Receptor beta; PDGF-R-beta; PDGFR; PDGFR-1; PDGFR-beta; PDGFR1; Platelet-derived growth factor receptor 1; Platelet-derived growth factor receptor beta; platelet-derived growth factor receptor, beta polypeptide
Gene Aliases: CD140B; IBGC4; IMF1; JTK12; KOGS; PDGFR; PDGFR-1; PDGFR1; PDGFRB; PENTT
UniProt ID: (Human) P09619
Entrez Gene ID: (Human) 5159
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